Single-cell analysis of the ventricular-subventricular zone reveals signatures of dorsal and ventral adult neurogenesis

The ventricular-subventricular zone (V-SVZ), on the walls of lateral ventricles, harbors largest neurogenic niche in adult mouse brain. Previous work has shown that neural stem/progenitor cells (NSPCs) different locations within V-SVZ produce subtypes new neurons for olfactory bulb. molecular signatures underlie this regional heterogeneity remain largely unknown. Here, we present a single-cell RNA-sequencing dataset revealing two populations NSPCs reside non-overlapping domains either dorsal or ventral V-SVZ. These differences gene expression were further validated using single-nucleus reference regionally microdissected and by immunocytochemistry RNAscope localization. We also identify subpopulations young have profiles consistent with origin. Interestingly, subset genes are dynamically expressed, but maintained, lineages. study provides novel markers territories to understand region-specific regulation neurogenesis.